equivalent to Drosophila 340
From Figure 2, 9 / micromolar / s in 10 mM NaCl, MONOMERIC bound empty binding ATP 500/s maximum?
From Figure 2, 4 / micromolar / s
To satisfy 60/s overall rate, they say hydrolysis must be at least 200/s
probably less than 40/s
Discussion says 75+/-10 /s
Later in discussion, REACTION 3, head release from ADP state is >300/s
110 /s from one method, 300/s from another; 150/s from model fitting. A third experiment indicated 400 /s., They seem to say both 150 and 200, but neither with too much confidence in the scheme, since the two methods don't agree, In companion paper, they cite the 110/s value only (p. 725, 2nd to last paragraph)
About 200/s, if using SCHEME 2 and assuming about 200/s for ADP release above.
hyperbolic fit to rate versus tubulin concentration
Sometimes they say 22C
No actually have to go back to Sadhu and Taylor JBC 1992
Unsure, but seems to be, 25 mM PIPES pH 6.9, 5 mM NaCl, 2 mM MgCl2, 1 mM EGTA, sometimes 25 mM KCl in 20 mM Tris pH 7.5, Seems to actually be 10 mM NaCl
The buffer is not PEM80
Some of their rate constants are derived from models. It is not clear which model is the best, and some of their experiments (e.g. ADP release) have disagreeing results from differing methods