Toolkit - Diagnostics

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Toolkit - Diagnostics by Mind Map: Toolkit - Diagnostics

1. Wolbachia diagnostics

1.1. Overview

1.1.1. Guideline: Quality assurance in mosquito production

1.1.2. DNA extraction

1.1.2.1. Stories

1.1.2.1.1. 7020. Extracting DNA from adult mosquitoes

1.1.2.2. Resources

1.1.2.2.1. SOP: Squash buffer extraction of DNA/RNA from mosquito samples

1.1.2.2.2. Template: Diagnostic data sheet

1.1.2.2.3. How to store squash buffer extraction plates

1.1.2.3. Troubleshooting

1.1.2.3.1. 5. Speeding up diagnostics

1.1.2.3.2. 323. Reducing BG trap collections.

1.1.2.4. Links to activities

1.1.2.4.1. Organise to outsource virus testing of blood

1.1.2.4.2. Diagnostics for field samples

1.1.2.4.3. Diagnostics for Mosquito Production

1.2. LAMP

1.2.1. Stories

1.2.1.1. 423. Switching to LAMP

1.2.2. Resources

1.2.2.1. 29. Testing for Wolbachia using LAMP

1.2.2.2. SOP: Diagnostic testing for Wolbachia in mosquitoes using qPCR (96-well)

1.2.3. Troubleshooting

1.2.3.1. Troubleshooting guide: LAMP

1.2.3.2. Guideline: Diagnostics QA

1.2.4. Links to activities

1.2.4.1. Test SOPs

1.2.4.2. Diagnostics for field samples

1.2.4.3. Diagnostics for Mosquito Production

1.3. qPCR

1.3.1. Stories

1.3.2. Resources

1.3.2.1. SOP: Testing for Wolbachia using qPCR (96-well)

1.3.2.2. SOP: Using the LightCycler 480's colour compensation function

1.3.2.3. How to test for Wolbachia density using qPCR (96-well)

1.3.3. Troubleshooting

1.3.3.1. Troubleshooting guide: qPCR

1.3.3.2. 184. After your qPCR has finished

1.3.3.3. 22. Interpreting qPCR results

2. Health checks

2.1. see Mosquito Production Toolkit

3. Vector competence

3.1. Overview

3.1.1. 6024. Testing the vector competence of new lines

3.2. Intrathoracic injection

3.2.1. Stories

3.2.1.1. 327. Injecting mosquitoes to test vector competence

3.2.2. Resources

3.2.2.1. SOP: Intrathoracic injection of mosquitoes with arboviruses

3.3. Spiked bloodfeeding

3.3.1. Stories

3.3.1.1. 326. Feeding viruses to mosquitoes

3.3.2. Resources

3.3.2.1. SOP: Viremic bloodfeeding of mosquitoes in PC3

3.4. Extraction and qPCR

3.4.1. Stories

3.4.1.1. 329. Checking the colony's vector competence

3.4.2. Resources

3.4.2.1. SOP: Diagnostic testing for viruses in mosquitoes using qPCR (96-well)

3.4.2.2. SOP: Quantification of Dengue, West Nile, Zika and Chikungunya Viral Titres by Plaque Assay

4. Core Data

4.1. see Data & Digital Toolkit

5. Insecticide Resistance

5.1. see Field Entomology Toolkit