Gilbert Lewis predicted in 1934 that deuterium may be essential for some life forms in his paper, The Biology of Heavy Water. He closed with, "It is not inconceivable that heavy hydrogen, which exists in small amounts in all natural water, may actually be essential to some plants or animals." However we have not been able to find any further experiments from Gilbert to support or contradict this question., http://en.wikipedia.org/wiki/Gilbert_N._Lewis, Lewis, G. N. (1934). THE BIOLOGY OF HEAVY WATER. Science (New York, N.Y.), 79(2042), 151-153. doi: 10.1126/science.79.2042.151., http://pubs.acs.org/doi/pdf/10.1021/ja01335a509
Dr. Koch's Code, https://github.com/stevekochscience/Deuterium-in-life-science/blob/master/Repeating%20Crumley/Water%20FTIR.R
My code, Installed Rcurl Package, Installed binom Package, Changed read.table to read.csv
Repeating Crumley, Original Experiment, http://research.iheartanthony.com/2011/08/24/repeating-crumley-et-al-1950/, http://research.iheartanthony.com/wp-content/uploads/2011/08/figure3-crumley.png, RC4: Time-Lapse Growth, http://research.iheartanthony.com/2011/11/15/rc4-time-lapse-growth/, Graphs of RC on figshare, http://figshare.com/figures/index.php/Tobacco_Seed_Growth_in_D2O
Even though deuterium is toxic in large quantities, have some life forms developed a need for it?, Effect of Deuterium Depeleted Water on Life, Introduction, http://research.iheartanthony.com/2011/08/16/effect-of-deuterium-depeleted-water-on-life/, Project Outline Yeast and E.Coli, http://research.iheartanthony.com/2011/12/13/project-outline-for-ddw-effects-on-microorgansims/, Havana Sample Pictures, Tap, http://research.iheartanthony.com/2011/12/09/ddw4-day-7-cropped-photos-of-tobacco-seeds/dsc_0014-4/, DDW, http://research.iheartanthony.com/2011/12/09/ddw4-day-7-cropped-photos-of-tobacco-seeds/dsc_0025-3/, DI, http://research.iheartanthony.com/2011/12/09/ddw4-day-7-cropped-photos-of-tobacco-seeds/dsc_0015-5/, Future Experiments, Escherichia coli, Yeast
FTIR, The DDW experiment is sensitive. The DDW will exchange with the surrounding atmosphere and become regular H2O (SMOW) over a period of time. SMOW: Standard Mean Ocean Water Has 156ppm of H2/H1 or 15mM, Deuterium Exchange? What is the rate of exchange? How accurate are the results because of the exchange that is always going on?, Need to accurately measure the amounts of D2O in H2O., Granted use by Dr. Sanjay Krishna courtesy of Stephen Myers, First try, Plastic cuvettes -- didn't work well at all, http://research.iheartanthony.com/2011/11/12/1st-try-at-ft-ir-spectroscopy/, Second try, Quartz cuvettes -- Da Bomb!! Worked Great!!, http://research.iheartanthony.com/wp-content/uploads/2011/12/ftir-water-types.png
all my research is reproducible.
Study of Protein Stability Studies done this summer REU Kenji, http://research.iheartanthony.com/2011/08/29/ovalbumin-catalase-and-kinesin-aggregation-data/, 1. Ovalbumin aggregates at ~75 C in DI water but not in D2O., https://docs.google.com/leaf?id=0B2DSwNF1eIqtYTE1MGI0MTYtNWI0ZS00NmYzLWI2ZWMtZDIwNmY5NTU1YTJj&hl=en_US, left is clear, right is aggregated, 2. Repeat of #1 with Catalase, So, stability is future work, but current work is related to the question "do cells specifically need deuterium?"