1. PART 2
1.1. BIOREATOR OPERATION
1.1.1. Operation -> The condition of functioning
1.1.2. Operation of bioreactor -> condition on how a bioreactor functioning
1.1.3. Function bioreactor -> Suitable environment in which an organism can effectively produce a target product
1.1.4. Target products : Cell biomass , Metabolite , Bioconversion Product
1.2. EFFECTS OF PHYSICAL FACTORS
1.2.1. OXYGEN
1.2.1.1. An essential element for aerobic growth
1.2.1.2. Will affects the product formation, by influence metabolic pathways and changing metabolic fluxes
1.2.1.3. Can adjusted by : Air Inlet Rate or Agitation Rate
1.2.2. NUTRIENT TRANSPORT
1.2.2.1. Rate of transport of a limiting nutrient
1.2.2.2. Increasing the rate of transport of limiting nutrient
1.2.3. TEMPERATURE
1.2.3.1. Affecting the performance of cells also affecting
1.2.3.2. When temperature ⬆️ above the optimum value, the maintenance requirements of cells
1.2.3.3. Above optimal temperature range -> growth rate decreases and thermal death may occur
1.2.4. FOAM FORMATION
1.2.4.1. Effect of foaming:-
1.2.4.1.1. Direct
1.2.4.1.2. Indirect
1.2.4.2. Occurs in bioprocess due to :- The introduction of gases and stabilised by proteins
1.2.4.3. Made up of liquid lamellas
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3. -
4. -
5. PART 1
5.1. Main Functions Of Bioreactor
5.1.1. Controlled environment
5.1.2. Growth of microorganism
5.1.3. Obtained a desired product
5.2. Bioreactor Operations
5.2.1. A good bioreactordesign should :
5.2.1.1. Improved peoductivity
5.2.1.2. Validition of desired parameters
5.2.1.3. Consistent and higher quality products
5.2.2. Design and mode of operation depends on on
5.2.2.1. The production of Organism
5.2.2.2. Optimum Condition Required For Desired Product Formation
5.2.2.3. Product Value
5.3. Effective Bioreactor
5.3.1. Control and positively influence the biological reaction
5.3.2. Prevent foreign contamination
5.3.3. Monoseptic conditions, optimal mixing with low uniform shear rates should be maintained throughout the process
5.4. -
5.5. Parameter In Bioreactor
5.5.1. Kinetics Parameter
5.5.1.1. Rate of Cell Growth
5.5.1.1.1. 4 Phase ( Lag phase , Log phase, deceleration phase and stationary phase)
5.5.1.1.2. Determine the amount of cell growth
5.5.1.2. Rate of Cell Death
5.5.1.2.1. Completion of cell growth
5.5.1.3. Specific Growth Rate
5.5.1.3.1. A quantitative measure of cell mass increase per unit of time
5.5.2. Physical Parameter
5.5.2.1. Rate of Agitation
5.5.2.1.1. Mixing process
5.5.2.1.2. Crucial for maximum production
5.5.2.1.3. One of the decisive factors of cultivated microorganisms
5.5.2.1.4. Scale-up & economy of aerobic biosynthesis systems
5.5.2.1.5. Inhibit the growth and activity of microorganisms by blocking the nutrients transfer and their consumption by disturbing cell activities.
5.5.2.1.6. Agitation will later effects:
5.6. Basic Operation Of Different Bioreactor
5.6.1. Batch Bioreactor
5.6.1.1. Closed Culture system
5.6.2. Continuos Bioreactor
5.6.2.1. Continuosly in steady state
5.6.3. Fed-Batch Bioreator
5.6.3.1. Not a closed system