Topic 3 Operation and Kinetics of Bioreactor Part 1

1. Main function of bioreactor 1.controlled environment 2.for growth microorganism 3.to obtained desired product

2. Design and mode of bioreactor based on : 1.The production of organism 2.Optimum condition 3. Product value 4.Scale of production

3. Basic operation of different bioreactors

3.1. Batch Bioreactor : -Closed culture system. -Contains an initial,limited amount of nutrient.

3.2. Continuous Bioreactors : -Continuously in steady state. -Amount of materials in is equal to the amount of product.

3.3. Fed-Batch Bioreactor : -Fed continuously -Sequentially with medium without removal of culture of fluid

4. Parameters in bioreactor Kinetic Parameter 1.Rate of cell cell growth 2.Rate of cell death 3.Rate of substrate consumption 4.Specific growth rate

5. Parameters in bioreactor Physical Parameter 1.Rate of agitation