Gastro-intestinal Tract
by Kai Yang Lee
1. [Solution] Manipulate the primary sequence of protein
1.1. How
1.1.1. By recombinant technologies
1.2. Why
1.2.1. To reduce propensity to unfold in acidic media
1.2.2. To lower their susceptibility to peptin-mediated digestion
2. [Solution] Replacement of phenylalanine & leucine residues analogues
2.1. Less recognition by pepsin in stomach
2.2. Why
2.2.1. Phenylalanine & leucine were hydrolyzed by pepsin in stomach mechanisms
3. Problems
3.1. GI tract has low pH environment (pH 1 -2.5, involving peptidase
3.2. Enzymes are potentially difficult to stabilize at GI since GI is a primary digestion site for proteins
3.3. Enzymes can be inactivated in a harsh environment occurs in stomach
4. [Solution] Gastro-resistant, polymer-coated tablets/ microparticles can be used
4.1. How
4.1.1. Prepared with cellulose acetate phthalate/ methacrylate copolymer
4.2. Why
4.2.1. Keep away from stomach digestion & coatings so it can be dissolved at higher pH at small intestine
5. Covalent conjugation to polymer of GI enzymes
5.1. How
5.1.1. Steric shielding
5.2. Why
5.2.1. Provides protections