1. The formal methionine
1.1. Carried by the transfer RNA in the p site
1.1.1. Joined the amino acid carried by the transfer RNA that jus entered the a site by a peptide bond
2. 70s ribosome
2.1. Peptidyl or P site
2.2. The acceptor or A site
2.3. Also have a third site called the exit or esight
2.3.1. Transfer RNAs are released
3. Initiating transfer RNA
3.1. carrying formyl methionine binds to the p site
3.2. Recognizes the next codon and carries the second amino acid then moves into the a site
4. Prokaryotic cells translation
4.1. initiated by formation of an initiation complex Consisting of:
4.1.1. 30s ribosomal
4.1.2. tRNA
4.1.3. messenger RNA
5. 50s ribosomal subunit
5.1. Joins the complex proteins called initiation factors are also involved but are not show
6. THE RIBOSOME
6.1. Now advances a distance or one codon
6.2. The transfer RNA that carried the formal methionine is released at the E site
6.2.1. A transfer RNA carrying the next amino acid now moves into the a site where the anticodon on the transfer RNA macthes the codon on the messenger RNA
6.3. Shifts down by a distance of one codon as
6.3.1. as shift occurs the two amino acids on the transfer RNA in the P site are transferred to the new amino acid
6.3.2. The second transfer RNA
6.3.2.1. Released from the E site
6.4. Continues to move along the messenger RNA
6.4.1. New amino acids are added to tge growing polypeptide chain
6.4.1.1. Elogation of polypeptide is terminated when a stop codon moves into the a site
6.4.1.1.1. A stop codon does not specify an amino acid
6.4.1.1.2. Also stop codon does not have a corresponding transfer RNA
6.5. Dissociates into the 30s and 50s subunits
6.5.1. The messenger RNA and protein are released