Organs-on-chip
por Blanca Raquel Gutiérrez Prieto
1. Cell culture
1.1. 2D cultures
1.2. 3D cultures
1.2.1. Spheroids
1.2.2. Organoids
2. Bioreaction design
2.1. Flow rate
2.2. Mixing
2.3. Modeling
2.3.1. Substrate and products
2.3.2. Specific growth rate
2.4. Process
2.4.1. CSTR
3. Microfluidics
3.1. Channels
3.1.1. Flow
3.1.2. Size
3.2. Materials
3.2.1. Biocompatibility
3.2.2. Porosity
3.3. Dimensions
3.4. Electrical stimuli
4. Characterization techniques
4.1. Fluorescence
4.1.1. Antibodies
4.2. Conductivity testing
4.3. Microscopy
4.3.1. Cell size
4.3.2. Uniformity
4.3.3. Time
4.4. Gene expression profile
4.5. Cell viability
4.5.1. Live/Dead assays
5. Applications
5.1. Neuronal system
5.2. Gut
6. Scaffolds
6.1. Hydrogels
6.1.1. GelMA
7. Microenvironment
7.1. Cell interactions
7.1.1. Signals
7.1.2. Density
7.2. Gradients
7.2.1. Oxygen
7.2.2. Nutrients