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Organs-on-chip by Mind Map: Organs-on-chip

1. Cell culture

1.1. 2D cultures

1.2. 3D cultures

1.2.1. Spheroids

1.2.2. Organoids

2. Characterization techniques

2.1. Fluorescence

2.1.1. Antibodies

2.2. Conductivity testing

2.3. Microscopy

2.3.1. Cell size

2.3.2. Uniformity

2.3.3. Time

2.4. Gene expression profile

2.5. Cell viability

2.5.1. Live/Dead assays

3. Bioreaction design

3.1. Flow rate

3.2. Mixing

3.3. Modeling

3.3.1. Substrate and products

3.3.2. Specific growth rate

3.4. Process

3.4.1. CSTR

4. Applications

4.1. Neuronal system

4.2. Gut

5. Microfluidics

5.1. Channels

5.1.1. Flow

5.1.2. Size

5.2. Materials

5.2.1. Biocompatibility

5.2.2. Porosity

5.3. Dimensions

5.4. Electrical stimuli

6. Scaffolds

6.1. Hydrogels

6.1.1. GelMA

7. Microenvironment

7.1. Cell interactions

7.1.1. Signals

7.1.2. Density

7.2. Gradients

7.2.1. Oxygen

7.2.2. Nutrients

7.3. Aerobic

7.4. Anaerobic