PURIFICATION OF PROTEINS

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PURIFICATION OF PROTEINS by Mind Map: PURIFICATION OF PROTEINS

1. isolation of protein from complex mixtures

2. INTRACELLULAR

2.1. 1. Removal of insoluble particles

2.2. * capture of solute in particulate free solution

2.3. 2. Isolation of the required -isolation carried out by solvent extraction

2.4. 3. Product purification

2.4.1. seperate contaminants by chromatography, cryst.graphy

2.5. 4. Incorporate of stabilizer

2.6. FINAL PRODUCT

2.6.1. liquid eg: subliciline

3. EXRACELLULAR

3.1. 1. Removal of cells

3.2. 2. Product purification

3.3. 3. Concentration of exract

3.4. 4. Solid / freeze dry etc

3.5. FINAL PRODUCT

4. ADVANTAGES

4.1. labour cost decrease

4.2. minimizes process time

4.3. bulk purchase

4.4. cost efficient

5. GRAS - generally regarded as safe

6. DOWNSTREAM PROCESSING

6.1. fermentation of submerged or semi- culture