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viro3002 by Mind Map: viro3002

1. the processes involved in the extraction of viral nucleic acids. (column based extraction method)

1.1. limitations

2. Antivirals

3. Theory of Prac

3.1. cell techniques

3.1.1. x. cultivation of cells, cell counting, sterilisation and disinfection techniques cell/tissue culture including composition and use of culture media and equipment used each cell line has its own characteristic growth pattern lab tq used for cultivation of cells Principles of BSC2 QC & troubleshooting cell cultures

3.1.2. x. establishment of a primary cell culture tissue culture and viral culture tq establishment of primary mammalian cell culture

3.1.3. cryopreservation of mammalian cells

3.1.4. x. recovery and growth of cells after cryogenic storage recovery and growth of cells after cryogenic storage

3.1.5. x. infection of a monolayer, enumerating viruses Viruses infecting cells, CPE, enumeration of viruses Infection of a cell monolayer with virus

3.1.6. x. immunofluorescent staining of infected cells IF staining to detect virus nfected cells theoretical aspects of IF staining to detect virus infections methodology of direct and indirect IF staining

3.1.7. x. diagnosis of viral infections-detecting virus, viral antigens or immune response to viral infections, Western blot Identification of viral infectiona (P1 detecting virus and viral antigens) Identification of viral infections (p2 Detecting immunological responses to viral infection) Harvesting virus from infected cells

3.2. molecular techniques

3.2.1. x. viral nucleic acid extractions - DNA and RNA the theory of polymerase chain reaction (PCR) amplification and be familiar with its applications to diagnostic virology. No amplification higher than expected mol. weight multiple, non specific amp products low yield the processes involved in the extraction of viral nucleic acids, specifically RNA. the principles behind and the process of RT-PCR RT

3.2.2. x. nucleic acid quantification

3.2.3. x. PCR amplification of viral genes and viral transcripts (including real-time PCR) Describe the hybridisation techniques, which may be used to identify virus strains. Dot blot southern blotting hybrid capture assay Understand the important molecular techniques used in the diagnosis of viral infections and be able to indicate the circumstances under which such techniques would be used influenza HA HPV principles of quantitative real-time PCR. Understand the differences between in “conventional” and real time quantitative PCR

3.2.4. x. electrophoresis principles and uses of gel-based analysis 300-10,000 bp theory of sequencing four di-deoxy nucleotides (coupled to a different fluorophore)

3.2.5. x. restriction enzyme digestion principles of restriction enzyme digestion

3.2.6. Be familiar with PC2 laboratory procedures

4. Theory

4.1. Viruses as pathogens

4.2. Immune responses to viral infection

4.3. Receptors

4.4. Transport

4.5. Zoonosis-vector borne diseases

4.6. Epidemiology and outbreak control

4.7. Electron Microscopy (cancelled?)

4.8. Diagnosis of viral infections (from sample to answer)

4.9. Emerging viruses

4.10. HIV/AIDS

4.10.1. Epidemiology and Evolution

4.10.2. Clinical aspects

4.11. Respiratory viruses/influenza

4.12. Herpesviruses

4.12.1. basics properties ubiquitous (highly successful) you cannot eliminate the virus common virion structure 4 distinct biological properties classification phases of infection Productive (lyitc) Latent (lysogenic) Reactivation

4.12.2. Alpha

4.12.3. Beta and Gamma

4.13. Viral infections and the fetus and newborn

4.14. Hepatitis (liver) unrelated

4.14.1. A and E

4.14.2. B, C and D

4.15. Gastroviruses

4.16. Dengue

4.17. Viruses and cancer

4.18. CNS disease

4.19. Gene therapy

4.20. Vaccines

5. Literature writing

5.1. writing style

5.1.1. spelling, grammar and expression Not too much waffle, not too much brevity - just right. Each sentence contains 1 (or at max, 2) ideas. Sentences >25 words should be split. Paragraphs = 1 idea where each paragraph is a part of a series to tell a bigger story.

5.1.2. layout and presentation

5.1.3. structure headings for clarity After gathering info, lay out the main points. Reorganise into logical flow. This is the skeleton. Each main point is one paragraph. Start with a general review of where your article is heading.

5.1.4. referencing purpose info trail give credit quality control ...also for "passing the buck"... :P common knowledge = no reference recently discovered/relatively unknown = reference

5.2. prereading techniques

5.3. task

5.3.1. Minireview: "Human cytomegalovirus disease in transplant recipients"

5.3.2. Due: 4pm Tues 23-9

5.3.3. 1500 words

5.3.4. <150 word abstract - not included in word count

5.4. Info gathering for task

5.4.1. 9 Sep lecture 16 Sep lecture basics properties classification phases of infection

5.4.2. Web HCMV HCMV allograft techniques (transfusion of antibody and immune cell serum)

5.4.3. Pubmed trawling (Comprehensive - all search results collated, for past 5 years. Search term: "transplant recipient" "cytomegalovirus") Categories

6. Fields Virology