Learn about quality control and troubleshooting in cell culture

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Learn about quality control and troubleshooting in cell culture by Mind Map: Learn about quality control and troubleshooting in cell culture

1. Contamination of culture

1.1. biological

1.2. chemical

2. QC

2.1. reagants, materials, equipment

2.1.1. reagants

2.1.1.1. Serum: FBS

2.1.1.2. Medium

2.1.1.3. water

2.1.1.4. pH (7-7.4)

2.1.1.5. Osmolality (osmometer)

2.1.1.6. missed component (new batch of medium)

2.1.1.7. check conc. of antibiotics

2.1.2. materials

2.1.2.1. glassware

2.1.2.1.1. wash

2.1.2.1.2. sterilisation

2.1.2.2. plastic consumables

2.1.2.2.1. dispose

2.1.3. equipment

2.1.3.1. incubators

2.1.3.2. BSC

2.2. cell lines

2.2.1. microbes

2.2.2. other cells

2.2.3. other experiments

2.3. microbial contamination

2.3.1. bacteria

2.3.2. yeast

2.3.3. fungus

2.3.4. mycoplasma

2.3.4.1. DNA stain

2.3.4.2. Direct culture

2.3.5. viruses

3. pH

3.1. red

3.2. pink

3.3. yellow