the theory of polymerase chain reaction (PCR) amplification and be familiar with its applications...

Get Started. It's Free
or sign up with your email address
Rocket clouds
the theory of polymerase chain reaction (PCR) amplification and be familiar with its applications to diagnostic virology. by Mind Map: the theory of polymerase chain reaction (PCR) amplification and be familiar with its applications to diagnostic virology.

1. Denature, Anneal, extension

2. -

2.1. high operator skill

2.2. susceptible to contamination

2.3. primer dimers

3. Pre-PCR

3.1. Post-PCR

4. PCR contamination control

4.1. separation of pre and post

4.2. use of physical aids

4.3. use of UV light and chemical means

4.4. aliquoted PCR reagants

4.5. incorporation of positive and negative controls (no template controls)

4.6. use of contamination control methods (for chemical and biochemical reactions)

5. Primer design

5.1. oligonucleotide length

5.1.1. radioactively labelled molecules or fluorescent dye molecules

5.2. terminal nucleotide

5.3. GC content and T_m

5.4. PCR product length

6. Confirmation of PCR products

6.1. restriction endonuclease digest

6.2. hybridisation with internal olionucleotide probe

6.3. direct sequencing of DNA

7. qPCR

7.1. HIV

7.1.1. progression of disease

7.1.2. effectiveness of HAART

7.1.3. monitor drug resistance

7.2. pathogenesis studies