Mention that it's definitely important for kinesin
And, given the crowded nature of cell, osmotic pressure in cell is important factor (can cite papers here)
Parsegian,Rand, Rau review (Meth Enz 1995), I want to convey same message as these guys / I wouldn't be averse to quoting them directly
Sidorova and Rau protein-DNA (PNAS, Biopolymers ~1996/2000?)
Fraunfelder Hydration Shell (2008?)
Both isotopes showed reduction in speed
D2O more substantial than O18
We'll discuss possible mechanisms for this effect, highlighting the change in solvent viscosity as a possible explanation
Cite, Gliding assay (Vale 19xx, kinesin home page), Maybe cite the viscosity paper (2010) here, definitely elsewhere in our paper of course), I remember seeing a Hackney 0-18 paper at Biophys 2010. Not sure if published though.
Cooke Lab work (osmotic/differing sizes)
Stabilized tubulin D2O, We have a paper (or more) showing that tubulin at 4C is more stable in D2O, Could also go further and cite that proteins in general are more stable in D2O (there is that "experts review" paper that I have, talking about vaccines specifically). Also there is the fruit fly D2O stability
Promoted MT polymerization D2O, I believe there were studies of MT polymerization in D2O showing that it proceeded more quickly. we have these refs
Bunch of things to cite for these Actin/Mysin and Tubulin papers. Probably like 6 total.