1. Colony fitness check
1.1. Introduction
1.2. Fitness and Wolbachia inheritance
1.2.1. Resources
1.2.1.1. Guideline: Quality assurance of mosquito production
1.2.1.2. SOP: Colony fitness check
1.2.1.3. link to Insecticide Resistance Toolkit
1.2.2. Case studies
1.2.2.1. 407. Isolating female mosquitoes
1.2.2.2. 3000. Counting larvae with a simple grid
1.2.3. Troubleshooting
1.2.4. Links to activities
1.2.4.1. Post-backcrossing health check of release colony [MP]
1.2.4.2. Pre-release health check of release colony [MP]
1.3. Insecticide Resistance
1.3.1. see Insecticide Resistance Toolkit
1.4. Vector competence
1.4.1. Resources
1.4.1.1. Guideline: Testing vector competence in Ae. aegypti
1.4.1.2. SOP: Propagation of enveloped viruses in C6/36 cells
1.4.1.3. SOP: Viremic bloodfeeding of mosquitoes
1.4.1.4. SOP: Intrathoracic injection of mosquitoes with arboviruses
1.4.1.5. SOP: Quantification of Dengue, West Nile, Zika and Chikungunya Viral Titres by Plaque Assay
1.4.2. Case studies
1.4.2.1. 326. Vector competence: spiked blood feeding
1.4.2.2. 327. Injecting mosquitoes to test vector competence
1.4.3. Troubleshooting
1.4.4. Links to activities
1.5. Innovation/Scale up
1.6. Exchange
2. Rearing for release
2.1. Introduction
2.1.1. 272. Insectary tour
2.2. Small cage rearing
2.2.1. Stories
2.2.1.1. 390. Collecting wildtype mosquitoes for outcrossing
2.2.1.2. 3012. How to assemble a BugDorm
2.2.1.3. 3051. Locally made rearing cages
2.2.1.4. 433. Contamination between colonies
2.2.2. Resources
2.2.2.1. SOP: Wolbachia colony maintenance (to be replaced by small cage rearing)
2.2.2.2. SOP: Small cage rearing (to replace Wolbachia colony maintenance)
2.2.2.3. How to label mosquito colonies
2.2.2.4. SOP: Determining egg hatch rate
2.2.2.5. Guidelines: Quality assurance of mosquito production
2.2.2.6. SOP: Egg drying and storage
2.3. Mass rearing
2.3.1. Stories
2.3.1.1. 278. Mass rearing in Yogyakarta
2.3.1.2. 280. Mass rearing in Yogyakarta, part 2
2.3.1.3. 3056. Acrylic trays for mass rearing
2.3.1.4. 451. Improving air quality for mass rearing
2.3.2. Resources
2.3.2.1. Guidelines: Mass rearing
2.3.2.2. SOP: Brushing and quantifying eggs
2.3.2.3. SOP: Hatching eggs for mass rearing
2.3.2.4. SOP: Mass rearing Ae. aegypti larvae
2.3.2.5. SOP: Mass rearing Ae. aegypti adults
2.4. need to add colony expansion to this toolkit?
2.5. Preparing mosquitoes for release > Eggs
2.5.1. Stories
2.5.2. Resources
2.5.2.1. Video: Ya-Hsun shows how to use a capsule filler
2.5.2.2. SOP: Egg capsule production
2.5.2.3. SOP: Brushing and quantifying eggs
2.5.2.4. Presentation: Egg capsules
2.6. Preparing mosquitoes for release > Adults
2.6.1. Stories
2.6.2. Resources
2.6.2.1. SOP: Preparing adult mosquitoes for remote release (cupping)
2.7. Preparing mosquitoes for release > Remote rearing
2.7.1. Stories
2.7.1.1. 337. Rearing mosquitoes for release
2.7.1.2. 338. Choosing a cup for rearing and releases
2.7.1.3. 315. Simple rearing for release
2.7.2. Resources
2.7.2.1. SOP: Preparing adult mosquitoes for remote release (cupping)
2.7.2.2. SOP: Egg capsule production
2.8. Tips & troubleshooting
2.8.1. Tips
2.8.1.1. 362. Vacuum hatching
2.8.1.2. 3000. Counting larvae with a simple grid
2.8.1.3. 3011. Sieving pupae with a bucket
2.8.1.4. 3055. A quick way to separate pupae
2.8.1.5. 3035. Measuring sugar in Falcon tubes
2.8.1.6. 3045. Finding a better sucrose feeding method
2.8.1.7. 3002. Weekly planning with a rainbow
2.9. Troubleshooting
2.9.1. 3044. Mouldy sucrose cups
2.9.2. 3050. Improving sucrose cups
2.9.3. 410. Rescuing over-fed larvae
2.9.4. 162. Dropping Wolbachia frequency in the release colony
2.9.5. 359. Investigating fail rates in adult release cups
3. Biobanking
3.1. see Field Entomology Toolkit
4. Bloodfeeding
4.1. Introduction
4.2. Blood supply and safety
4.2.1. Resources
4.2.1.1. How to ensure quality and safety of blood
4.2.1.2. SOP: Safe blood handling and disposal
4.2.2. Stories
4.2.2.1. 331 - Sourcing human blood
4.2.2.2. 342 - Blood feeding permits
4.2.2.3. 241 - Safe bloodfeeding in Colombia
4.2.2.4. 164 - Screening mosquitoes for viruses before release
4.3. Beeswax feeders
4.3.1. Resources
4.3.1.1. SOP: Bloodfeeding using beeswax pots
4.3.2. Stories
4.3.2.1. 368 - Bloodfeeding using beeswax pots
4.4. Other bloodfeeding methods
4.4.1. Resources
4.4.1.1. SOP: Bloodfeeding using artifical methods (Hemotek system)
4.4.2. Stories
4.4.2.1. 313. Feeding mosquitoes with an artificial system (Hemotek)
4.4.2.2. 326. Feeding viruses to mosquitoes (glass membrane feeders)
5. Insecticide resistance
5.1. see Field Entomology Toolkit
6. Processing field samples
6.1. Intro
6.2. Processing eggs
6.2.1. Stories
6.2.1.1. 390. Collecting wildtype mosquitoes for outcrossing
6.2.1.2. 3012. How to assemble a BugDorm
6.2.1.3. 214 Creating a release colony for Townsville
6.2.2. Resources
6.2.2.1. SOP: Mosquito collection using ovitraps
6.2.2.2. SOP: Shipping biological samples to Australia
6.2.2.3. Form: Shipping biological samples to Australia
6.2.2.4. SOP: Small cage rearing
6.2.3. Troubleshooting
6.2.3.1. 271. Improving egg hatch rate
6.2.3.2. 3051. Locally-made rearing cages
6.2.4. Links to activities
6.2.4.1. Rear Wolbachia mosquitoes from WMP [MP]
6.2.4.2. Entomological survey [FE]
6.2.4.3. Insecticide resistance survey [FE]
6.2.4.4. Collect wildtype mosquitoes for backcrossing [FE]
6.2.4.5. Monitoring [FE]
6.3. Processing adult mosquito samples
6.3.1. Stories
6.3.1.1. 264. Processing BG trap samples
6.3.2. Resources
6.4. Mosquito identification
6.4.1. Stories
6.4.1.1. 375 Identifying mosquitoes under the microscope
6.4.2. Resources
6.4.2.1. SOP: Identifying Ae aegypti adults
6.4.2.2. SOP: Identifying Ae aegypti larvae
6.4.2.3. Mosquito identification key (Reuda 2004)
6.4.2.4. SOP: Using the Mosquito ID application
6.5. Innovation/Scale up
6.5.1. Mosquito ID keys
6.5.2. How to ID mosquitoes efficiently at scale? Is it necessary?
6.5.3. QA for ID
7. Mosquito releases
7.1. see Field Entomology Toolkit
8. Producing a Wolbachia colony
8.1. Introduction
8.2. Stories
8.2.1. 214. Creating a release colony for Townsville
8.2.2. 390. Collecting wildtype mosquitoes for outcrossing
8.2.3. 433. Contamination between colonies
8.3. Resources
8.3.1. Guideline: Backcrossing and outcrossing a Wolbachia colony
8.3.2. SOP: Wolbachia colony maintenance (to be replaced by small cage rearing)
8.3.3. SOP: Small cage rearing (to replace Wolbachia colony maintenance)
8.3.4. How to calculate the number of eggs required for backcrossing
8.3.5. SOP: Establishing a wildtype colony (to be replaced by Establishing a colony)
8.3.6. SOP: Establishing a colony (to replace Establishing a wildtype colony)
8.3.7. How to label mosquito colonies
8.4. Troubleshooting
8.4.1. Troubleshooting guide: Backcrossing