Chemicals and recipes

1. 1000x Carb

1.1. 50 mg/mL carb

1.2. Dissolve in 1:1 water:ethanol

1.3. No filtration necessary

1.4. Doesn't keep forever, test/remake occasionally

2. Media

2.1. LB

2.1.1. 10

2.1.2. 10

2.1.3. 5

2.2. TB

2.3. 2YT

2.3.1. 16

2.3.2. 5

2.3.3. 10

3. grams / liter

4. Also needs glycerol, it's included in Baker powdered TB

5. Chemicals

5.1. HEPES

5.2. HCl

5.3. Acetonitrile

5.3.1. Shortage

5.3.1.1. Currently paying $1000 per 16 L case

5.3.2. MW

5.4. Tris-Hcl

5.4.1. MW

5.4.2. pKa

5.5. Tris-base

5.5.1. MW

5.5.2. pKa

5.6. NaCl

5.6.1. MW

5.7. Biotin

6. 500x Arabinose (0.5% w/v)

6.1. Subtopic 1

7. 1000x Chlor

8. 1000x IPTG

9. IPTG+Arabinose induction mix

9.1. 100 uL per 5 mL

9.1.1. For 100 uL

9.1.1.1. 5 uL IPTG

9.1.1.2. 10 uL Arabinose

9.1.1.3. 85 uL water

9.1.2. For 1 mL

9.1.2.1. 50 uL IPTG

9.1.2.2. 100 uL arabinose

9.1.2.3. 850 uL water

9.2. 20 uL per 5 mL

9.2.1. For 20 uL

9.2.1.1. 5 uL IPTG

9.2.1.2. 10 uL arabinose

9.2.1.3. 5 uL water

9.2.2. For 1 mL

9.2.2.1. 250 uL IPTG

9.2.2.2. 500 uL arabinose

9.2.2.3. 250 uL water

10. 1000x Kan