Chemicals and recipes
by Matthew D. Smith

1. 1000x Carb
1.1. 50 mg/mL carb
1.2. Dissolve in 1:1 water:ethanol
1.3. No filtration necessary
1.4. Doesn't keep forever, test/remake occasionally
2. Media
2.1. LB
2.1.1. 10
2.1.2. 10
2.1.3. 5
2.2. TB
2.3. 2YT
2.3.1. 16
2.3.2. 5
2.3.3. 10
3. grams / liter
4. Also needs glycerol, it's included in Baker powdered TB
5. Chemicals
5.1. HEPES
5.2. HCl
5.3. Acetonitrile
5.3.1. Shortage
5.3.1.1. Currently paying $1000 per 16 L case
5.3.2. MW
5.4. Tris-Hcl
5.4.1. MW
5.4.2. pKa
5.5. Tris-base
5.5.1. MW
5.5.2. pKa
5.6. NaCl
5.6.1. MW
5.7. Biotin
6. 500x Arabinose (0.5% w/v)
6.1. Subtopic 1
7. 1000x Chlor
8. 1000x IPTG
9. IPTG+Arabinose induction mix
9.1. 100 uL per 5 mL
9.1.1. For 100 uL
9.1.1.1. 5 uL IPTG
9.1.1.2. 10 uL Arabinose
9.1.1.3. 85 uL water
9.1.2. For 1 mL
9.1.2.1. 50 uL IPTG
9.1.2.2. 100 uL arabinose
9.1.2.3. 850 uL water
9.2. 20 uL per 5 mL
9.2.1. For 20 uL
9.2.1.1. 5 uL IPTG
9.2.1.2. 10 uL arabinose
9.2.1.3. 5 uL water
9.2.2. For 1 mL
9.2.2.1. 250 uL IPTG
9.2.2.2. 500 uL arabinose
9.2.2.3. 250 uL water