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Chemicals and recipes by Mind Map: Chemicals and recipes

1. Chemicals

1.1. HEPES

1.2. HCl

1.3. Acetonitrile

1.3.1. Shortage

1.3.1.1. Currently paying $1000 per 16 L case

1.3.2. MW

1.4. Tris-Hcl

1.4.1. MW

1.4.2. pKa

1.5. Tris-base

1.5.1. MW

1.5.2. pKa

1.6. NaCl

1.6.1. MW

1.7. Biotin

2. 500x Arabinose (0.5% w/v)

2.1. Subtopic 1

3. 1000x Chlor

4. 1000x IPTG

5. IPTG+Arabinose induction mix

5.1. 100 uL per 5 mL

5.1.1. For 100 uL

5.1.1.1. 5 uL IPTG

5.1.1.2. 10 uL Arabinose

5.1.1.3. 85 uL water

5.1.2. For 1 mL

5.1.2.1. 50 uL IPTG

5.1.2.2. 100 uL arabinose

5.1.2.3. 850 uL water

5.2. 20 uL per 5 mL

5.2.1. For 20 uL

5.2.1.1. 5 uL IPTG

5.2.1.2. 10 uL arabinose

5.2.1.3. 5 uL water

5.2.2. For 1 mL

5.2.2.1. 250 uL IPTG

5.2.2.2. 500 uL arabinose

5.2.2.3. 250 uL water

6. 1000x Kan

7. 1000x Carb

7.1. 50 mg/mL carb

7.2. Dissolve in 1:1 water:ethanol

7.3. No filtration necessary

7.4. Doesn't keep forever, test/remake occasionally

8. Media

8.1. LB

8.1.1. 10

8.1.2. 10

8.1.3. 5

8.2. TB

8.3. 2YT

8.3.1. 16

8.3.2. 5

8.3.3. 10

9. grams / liter

10. Also needs glycerol, it's included in Baker powdered TB