Copy of Bioinformatics
by Farisya Ailin
1. What is Bioinformatics?
1.1. Application of computational tools on molecular data
1.1.1. Acquire
1.1.2. Analyze
1.1.3. Visualize such data
1.2. Molecular data
1.2.1. Sequence of DNA
1.2.2. cDNA
1.2.3. Protein
2. What is BLAST
2.1. Abbreviation for Basic Local Alignment Search Tools
2.2. A tool for searching gene or protein sequence databases for related genes interest
2.3. Alignments between the query sequence and any given database sequence, allowing for mismatches and gaps, indicate their degree of similarity
3. What bioinformatics can do?
3.1. Pattern recognition
3.1.1. A particular sequence or structure has been seen before' and that a particular characteristic can be associated with it
3.2. Prediction
3.2.1. From a sequence we can predict the structure and a function
4. Why do we use Bioinformatics?
4.1. Store biological information (database)
4.2. Retrieve/compare gene sequences
4.3. Predict function of unknown genes/proteins
4.4. Search for previously known function of a gene
4.5. Compare data with other researchers
4.6. Compile/distribute data for other researchers
5. How rDNA technology connected to Bioinfo?
5.1. DNA extraction
5.1.1. From any organisms
5.1.2. Verify the DNA through AGE
5.1.3. Agarose percentage 1/DNA size
5.2. PCR
5.2.1. Amplify targeted DNA sequence using a pair of primers
5.2.2. Verify amplified DNA fragments through AGE
5.3. DNA sequencing
5.3.1. Verify the amplified DNA fragments from PCR through DNA sequencing
5.3.2. Use automated DNA sequencing approach
5.4. Bioinformatics
5.4.1. Interpret message in the DNA sequence
5.4.2. Usage of BLAST program provided by NCBI
5.4.3. Results shown as BLAST output