Requirement

1. Donor screening

1.1. 18 to 60 years old and weights more than 50kg

1.2. donation frequency

1.3. standard laboratory test

1.4. blood donation forms.

1.5. Before and after donating

2. Blood collection record

2.1. Equipment assemble like syringe, multiple sample needles and tourniquet.

2.2. Identify and prepare the patient

2.3. Prepare the site

2.4. Donor and patient record log book

3. Processing

3.1. Needle, Rubber tube and satellite bag

3.2. Anticoagulants / preservatives

4. Preparation of blood

4.1. Hgb < 7 g/dl in an asymptomatic patient.

4.2. Hgb <10 g/dl in cases of an increased risk of ischemia.

4.3. Blood cross matching

4.4. light spin - short with low rpm(2000)

4.5. heavy spin - longer spin with high rpm(3500)

5. Preparation of plasma

5.1. Human blood plasma which is frozen within 8hrs less than -18 degree celcius.

5.2. Vacutainer tubes containing anticoagulant (EDTA)

5.3. Serological pipettes of appropriate volume (sterile)

5.4. Centrifuge tubes and benchtop centrifuge

5.5. Cryovials

5.6. Stored at 1to 6 degree celcius and transfused within 24 hours.

5.7. Needs to be ABO compatible

6. Labelling

6.1. ISBT labelling