Cells & Molecular Genetics

Secondary 3 biology (topics: cells & molecular genetics)

Get Started. It's Free
or sign up with your email address
Rocket clouds
Cells & Molecular Genetics by Mind Map: Cells & Molecular Genetics

1. different viewing perspectives

1.1. longitudinal section

1.1.1. cutting along the length of the cell

1.2. transverse section

1.2.1. cutting across the length of the cell

2. types

2.1. typical plant cell

2.1.1. has cell wall made of cellulose fully permeable absent in animal cells chloroplasts oval structures containing chlorophyll which absorbs light energy to carry out photosynthesis tonoplast encloses vacuole store substances withing the cell

2.2. typical animal cell

2.2.1. has nucleus controls cell activities, e.g. essential for cell division contains cell surface membrane aka plasma membrane partially permeable controls the substances entering and leaving the cell cytoplasm site of most cell activities contains specialised membrane-bound structures calles organelles contains

2.3. specialised cells

2.3.1. what is differentiation? process by which a cell becomes specialised for a specific function

2.3.2. examples red blood cell (RBC) haemoglobin in cytoplasm of RBC -> transports oxygen from lungs to all parts of the body has no nucleus -> can carry more haemoglobin and thus more oxygen circular biconcave shape (thinner central portions) -> increases surface area to volume ratio -. increases rate of diffusion of oxygen into and out of the cell flexible and elastic -> able to turn bell-shaped to allow the cell to squeeze through tiny capillaries xylem vessels long hollow tubes from the root to the leaves narrow and do not have cross walls or protoplasm to obstruct water and mieral salt flow (reduce resistance) through the lumen walls thickened with lignin -> prevent the vessel from collapsing vessels clumped together -> making lumen even stronger, keeping the plant upright root hair cell

2.4. long & narrow protrusion -> increases surface area to volume ratio, allowing the root hair cell to absorb water and mineral salts at a faster rate

2.5. differences

2.5.1. plant cell cell wall present chloroplast present centrioles absent a large central vacuole

2.5.2. animal cell cell wall absent chloroplast absent centrioles present (involved in cell division) numerous small vacuoles

3. are the

3.1. building blocks of life

3.1.1. too small to be seen by the naked eye so we use a light microscope electron microscope

3.2. simplest units that exhibit characteristics of life

4. cells

4.1. tissue

4.1.1. organ organ system organism several related organs working together to carry out a particular function e.g. digestive system, respiratory system different tissues working together to carry out a specific function different tissues may be untied to form an organ e.g. stomach: digests food and is made up of glandular tissue, muscular tissue and nervous tissue e.g. leaf: made up of several types of tissues, namely the mesophyll tissue, xylem tissue & phloem tissue

4.1.2. a group of similar cells which work together to perform a certain function

4.1.3. cells of the same type e.g. muscle & epithelial tissue in animals, epidermis & mesophyll tissue in plants

4.1.4. complex tissues are tissues made up of several different cell types e.g. nervous tissue (sensory neurones, relay neurones, motor neurones) & blood tissue (RBCs, WBCs, platelets, plasma) in animals, vascular tissue (xylem & phloem, where phloem consists of sieve tubes, sieve plate & companion cells) in plants

4.2. specialised for a specific function

5. Molecular Genetics

5.1. deoxyribonucleic acid (DNA)

5.1.1. organisation each DNA molecule consists of two anti-parallel strands (the two strands run in opposite directions) twisted around each other to form a double helix a molecule of DNA is wrapped around proteins (histones) to form a single chromatin thread during cell division, the chromatin threads coil & condense more tightly to form chromosomes inside the cell nucleus

5.1.2. what is DNA? molecule that carries genetic information made up of two anti-parallel polynucleotide strands bases on one strand form bonds with the bases on the other strand according to the rule of complementary base pairing one coding strand and one non-coding strand double helix structure made up of nucleotides which are made up of can be joined together to form polynucleotides

5.2. genes

5.2.1. a segment of DNA that codes for the synthesis of a single polypeptide determined by the nucleotide sequence in the gene 3 nucleotides in a gene form a codon and each codon codes for one amino acid total of 64 (4 to the power of 3) different possible codons genetic code states which amino acid each codon codes for important pointers in ribonucleic acid (RNA), thymine is replaced by uracil

5.2.2. what happens when the nucleotide sequence in a gene is altered? termed as a gene mutation gene mutation may or may not lead to a change in protein product e.g. changing GUU to GUC will not change the product formed but changing GUU to AUU will change the product formed change in protein may or may not lead to an observable phenotype e.g. of gene mutations albanism: mutation in the the gene causes an absence or defect in the enzyme that produces the pigment melanin sickle-cell anaemia: mutation in the gene causes the protein product to differ from the normal protein by a single amino acid, causing RBC to become sickle DNA -> amino acid (polypeptide) -> folds to form a protein

5.2.3. how proteins are made from genes DNA to messenger RNA (mRNA): process - transcription, location - nucleus process biological molecules involved DNA is the template for mRNA synthesis mRNA to polypeptide: process - translation, location - cytoplasm process mRNA is the template for polypeptide synthesis

5.2.4. comparison between DNA and RNA molecules DNA responsible for storing and transferring genetic information deoxyribose sugar double-stranded nitrogenous bases: guanine, cytosine, adenine, THYMINE bigger; longer chain of nucleotides ratios of A:T & G:C = 1:1 always present RNA directly codes for amino acids and acts as a messenger between DNA and ribosomes to make proteins ribose sugar single-stranded nitrogenous bases: guanine, cytosine, adenine, URACIL smaller; shorter chain of nucleotides no fixed ratio only made when needed

5.3. transferring genes between organisms

5.3.1. genetic engineering refers to the manipulation of an organism's genetic material

5.3.2. involves the transfer of genes from one organism to another

5.3.3. done by the use of a vector molecule a vector molecule is a molecule that is used to carry a gene or genes from one organism to another plasmids (circular DNA) from bacteria are commonly used as vectors

5.3.4. process isolate the desired gene desired gene is 'cut' using a restriction enzyme, creating complementary sticky ends at the ends of the desired gene the bacterial plasmid is 'cut' by the same restriction enzyme as the desired gene, creating complementary sticky ends in the bacterial plasmid as well insert the gene into the vector DNA the desired gene is inserted into the bacterial plasmid held together using DNA ligase which acts as a glue for the sticky ends of the desired gene and the bacterial plasmid forms a recombinant plasmid insert the recombinant plasmids into bacteria the recombinant plasmid is too large to fit through the cell membrane of the bacterium the recombinant plasmid and the bacterium are given a heat shock the pores on the cell surface membrane open temporarily for the plasmid to enter forms a transgenic bacterium

5.3.5. producing human insulin background: type 1 diabetes is caused by the inability of the islet of Langerhans to produce sufficient insulin mass production of insulin for type 1 diabetes patients was made possible through the use of genetic engineering the human insulin gene is transferred to bacterial cells that are able to express th gene the product (insulin) can then be harvested

5.3.6. large scale production of human insulin transgenic bacteria need to be burst open in order to extract the human insulin that is produced in bacteria in order to produce large amounts of human insulin, large amounts of transgenic bacteria needs to be cultured done through the use of large sterile containers called fermenters essential components of a fermenter nutrient broth cooling kacket pH controller aeration system

5.3.7. other applications of genetic engineering creation of transgenic plants that are resistant to herbicides pests gene therapy: healthy genes from a person can be transferred to the cells of another person with defective genes note: genes can be transferred between organisms of different species and between organisms of the same species e.g. transfer of a pest-resistant gene from wild wheat plants to common wheat plants that are grown as crop

5.3.8. selective breeding vs genetic engineering selective breeding organisms involved in selective breeding must be closely related or of the same species there is a possibility that defective genes will be transmitted to the offspring slow process that involves several generations less efficient as organisms grow more slowly and may require more food genetic engineering genes from an organism can be inserted into non-related species or different species selection of genes before transfer eliminates risk of transferring a defective gene a process which uses individual cells that reproduce rapidly in a small container in a laboratory more efficient as transgenic organisms grow faster and may require less food

5.4. effects of genetic engineering on society

5.4.1. advantages low cost production of medicines drugs such as human insulin become more affordable production of crops that grow in extreme conditions farmers are able to grow crops in environmental conditions that are unfavourable for cultivating most crops development of pesticide-resistant crops use of costly pesticides that may damage the environment is reduced development of foods designed to meet specific nutritional goals nutritional quality of food is improved

5.4.2. disadvantages economic hazards if prices of genetically (GM) crop seeds are not regulated, poorer farmers may not benefit from the technology while their richer competitors will continue to get richer through the technology environmental hazards GM crops that produce insect toxins may result in the loss of insect biodiversity social and ethical hazards GM may lead to class distinctions and religious disputes health hazards genes that code for antibiotic resistance may be accidentally incorporated into bacteria that cause human diseases