Molecular Methods

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Molecular Methods by Mind Map: Molecular Methods

1. DNA Isolation

1.1. Phenol & Chloroform

1.1.1. Steps

1.2. Protein binding w/ Salts

1.3. Platform or Carrier

2. Analyzing Nucleic Acids

2.1. UV Spectrophotometry

2.1.1. Questions

2.1.1.1. Peak absorption of nucleic aics?

2.1.1.2. How does it work?

2.1.1.3. What is it looking for exactly?

2.1.1.4. How is purity of sample analyzed?

2.2. Agarose Gel Electrophoresis

2.2.1. Questions

2.2.1.1. What is this method good for?

2.2.1.2. Which type of RNA is usually sampled?

2.2.1.3. What moves the DNA across agarose gel?

2.2.1.4. Gel is stained with?

2.2.1.5. Good quality sample shows what features?

3. General use for DNA isolation?

4. Methods used to isolate depend on what?

5. Common sources of DNA?

6. Why does DNA precipitate in alcohol?

7. Nucleic Acid Hybridization

7.1. FISH

7.1.1. Questions

7.1.1.1. Generally - what is it used for?

7.1.1.2. Which cellular components are analyzed?

7.1.1.3. Overview - steps of FISH?

7.1.1.4. By what mechanism does it work?

7.1.1.5. What does it detect?

7.2. CGH

7.2.1. Questions

7.2.1.1. When is this method used?

7.2.1.2. Which cellular components are analyzed?

7.2.1.3. What does it detect

7.2.1.4. Color indications?

7.3. aCGH

7.3.1. Questions

7.3.1.1. Which cellular components are analyzed?

7.3.1.2. Main difference from CGH?

7.3.1.3. What does it detect?

7.3.1.4. Color indications?

7.4. MicroArray

7.4.1. Questions

7.4.1.1. When is this method used?

7.4.1.2. What probes are used?

7.4.1.3. Main benefit of this method?

7.4.1.4. Which cellular components are analyzed?

7.4.1.5. How is "macroarray" different?

7.4.1.6. Color indications?

7.5. Northen Blotting

7.5.1. Questions

7.5.1.1. What does it detect?

7.5.1.2. When is this method used?

7.5.1.3. Which cellular components are analyzed?

7.6. Southern Blotting

7.6.1. Questions

7.6.1.1. When is this method used?

7.6.1.2. What does it detect?

7.6.1.3. What is required for this method (but NOT for Northern Blot)?

7.6.1.4. Which cellular components are analyzed?