Molecular Methods II

Medical School topic overview

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Molecular Methods II by Mind Map: Molecular Methods II

1. UNKNOWN MUTATIONS

1.1. SSCP

1.1.1. Used for what purpose?

1.1.2. What can it detect?

1.1.3. Limitations

1.1.4. How are results analyzed?

1.2. PTT

1.2.1. Used for what purpose?

1.2.2. How are results anaylzed?

1.2.3. What can it detect?

2. UNKNOWN + KNOWN

2.1. Automated Sequencing

2.1.1. How does it work?

2.1.2. What can it detect?

2.1.3. How are results analyzed?

2.1.4. Main advantages of this method?

2.2. Next Generation Sequencing

2.2.1. Applications of NGS

3. Amplification by PCR

3.1. RT-PCR

3.2. Q/A

3.2.1. Used for what purpose?

3.2.2. Requisites?

3.2.3. General Steps

3.2.4. Samples taken from where?

3.2.5. Pros/Cons

3.2.6. How are results analyzed?

3.2.6.1. Included

3.2.6.2. Included

3.2.6.3. Excluded

4. Example of dNTPs:

5. KNOWN MUTATIONS

5.1. ARMS

5.1.1. Q/A

5.1.1.1. Used for what purpose?

5.1.1.2. Requisites?

5.1.1.3. How are results analyzed?

5.2. RFLP

5.2.1. Q/A

5.2.1.1. Used for what purpose?

5.2.1.1.1. Materials

5.2.1.1.2. Personel

5.2.1.1.3. Services

5.2.1.1.4. Duration

5.2.1.2. Requisites?

5.2.1.3. Samples taken from where?

5.2.1.4. General steps

5.2.1.5. How are results analyzed?

5.3. Multiplex PCR

5.3.1. Q/A

5.3.1.1. Used for what purpose?

5.3.1.2. Requisites?

5.4. Real Time PCR

5.4.1. Q/A

5.4.1.1. Used for what purpose?

5.4.1.2. What is unique about this one?