Identification method of microorganisms
by Nur Ezzati Rosli

1. Gram stain
1.1. Gram positive : Purple
2. Stain bacteria with waxy material
3. Endospore staining
3.1. Schaeffer-Fulton endospore stain
3.1.1. Use a mordant and stain carbolfuchsin to coat the flagella
3.2. Endospore appear green within pink cells
4. Biochemical tests
4.1. To verify its metabolic activity
4.1.1. - Phenol Red broth - Casease Test - Gelatin Test - Lipase Test - Starch hydrolysis - Motility test - Catalase test
5. Numerical taxonomy
5.1. Computer assisted taxonomy
6. Differential staining
6.1. Gram negative : Pink
6.2. Acid-fast stain
6.3. Negative staining for capsules
6.3.1. Use india ink to provide a contrasting background then stain with safranin