Identification method of microorganisms

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Identification method of microorganisms by Mind Map: Identification method of microorganisms

1. Endospore staining

1.1. Schaeffer-Fulton endospore stain

1.1.1. Use a mordant and stain carbolfuchsin to coat the flagella

1.2. Endospore appear green within pink cells

2. Biochemical tests

2.1. To verify its metabolic activity

2.1.1. - Phenol Red broth - Casease Test - Gelatin Test - Lipase Test - Starch hydrolysis - Motility test - Catalase test

3. Numerical taxonomy

3.1. Computer assisted taxonomy

4. Differential staining

4.1. Gram stain

4.1.1. Gram positive : Purple

4.1.2. Gram negative : Pink

4.2. Acid-fast stain

4.2.1. Stain bacteria with waxy material

4.3. Negative staining for capsules

4.3.1. Use india ink to provide a contrasting background then stain with safranin

4.4. Flagella staining

5. Morphology characteristics

5.1. - cell type (prokaryote or eukaryote) - shape and size - cellular grouping (chain, clump) - external structures (flagella, motility) - internal structures (inclusion granules)