HSC biotechnology

1. genetic technologies

1.1. recombinant DNA e.g. E.coli

1.1.1. transgenic species e.g. Bt cotton, Golden Rice

1.1.2. gene therapy

1.1.2.1. direct

1.1.2.2. stem cell

1.1.3. medical uses

1.1.3.1. recombinant DNA vaccine e.g. Hep B

1.1.3.2. xenotransplantation e.g. pig

1.1.3.3. knock out mice

1.2. GMO

1.3. DNA splicing

1.4. DNA sequencing and profiling

1.4.1. PCR

1.4.1.1. 1.Denaturation 2.Annealing 3.Extension

1.4.2. gel electrophoresis

1.5. cloning

1.5.1. gene e.g. insulin

1.5.1.1. 1.Isolation 2.Ligation 3. Transformation 4.Replication

1.5.2. whole organism e.g. Dolly

1.5.2.1. SCNT

1.5.2.2. artificial embryo twinning

1.5.2.3. horticultural cloning

2. reproductive technologies

2.1. selective breeding e.g. Fresian bull x Jersey cow

2.2. artificial insemination e.g. ToyStory

2.3. artificial pollination

2.3.1. self pollination

2.3.2. cross pollination

2.4. IVF