Prokaryotic DNA Replication

1. Single stranded binding proteins stabilize the DNA

2. Dna C loads Dna B (helicase) along the 13-mers

3. Lagging strand (Okazaki fragments) in 3’-5’ direction

4. DNA Pol III synthesizes new strands along the RNA primers

4.1. Continuous strand in 5’-3’ direction

5. DNA Pol III binds the helicase and DNA; synthesizes a short RNA primer

6. Initiation

6.1. Ori C consists of 9-mers and 13-mers

6.2. Dna A proteins bind the 9-mers, leading to separation of 13-mers

7. Elongation

8. Termination

8.1. DNA Pol III detaches from the DNA at the terA-terF sequences

8.2. Tus (replication terminator protein) ends replication

8.3. DNA Pol I removes RNA primers and adds DNA segments in their place

8.4. Ligase joins the gaps