Chromatography

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Chromatography af Mind Map: Chromatography

1. Applications

1.1. Qualitative Analysis

1.2. Quantitative Analysis

1.3. Ion-Exclusion Chromatography

2. Chromatography theory

2.1. Rate theory

2.2. Plate theory

3. Biphasic systems

3.1. Stationary phase

3.1.1. Solid/liquid supported on a solid/gel or a small solid particle with micro porous surface

3.1.1.1. Gas/Gas-solid

3.1.1.2. Liquid/liquid-solid

3.2. Mobile phase

3.2.1. Gas/liquid carriers of a mixture components. The rate of movement depends on solubility in solvent systems

3.2.1.1. Gas/gas-liquid

3.2.1.2. Liquid/Liquid-liquid

4. Types of chromatographic methods

4.1. Based on quantity

4.1.1. Microscale/lab assay such as TLC/PC

4.1.2. Medium scale/analytical assay i.e. column flow

4.1.3. Large scale/commercial i.e. GSC

4.2. Based on solvent direction

4.2.1. Upward ascending

4.2.2. Downward descending

4.2.3. Horizontal transverse dial

5. Column chromatography

5.1. Gas-liquid chromatography (GLC)

5.2. Gas-solid

5.2.1. GC-MS

5.3. Liquid chromatography (LC)

5.3.1. Liquid-liquid

5.3.2. Liquid-solid

5.3.3. Ion-exchange

5.3.3.1. Inorganic-Ion Chromatography

5.3.3.2. Single-Column Ion Chromatography

5.3.4. Size-exclusion chromatography

5.3.5. Affinity chromatography

5.3.6. Chiral chromatography

5.3.7. Electrochromatography

5.4. Supercritical fluid chromatography

6. Planar chromatography

6.1. Thin-layer chromatography

6.1.1. Qualitative TLC

6.2. Paper chromatography /PC

6.2.1. Two-Dimensional Planar Chromatography

6.3. Partition chromatography

7. Characteristics

7.1. Adsorption: column include thin layer and ion-exchange chromatography

7.2. Solvent systems: Stable composition and solvent choice/definite solubility