HPLC: Thermo Scientific Orbitrap Fusion Lumos Tribrid Mass Spectrometer

1. System works with:

1.1. the brightest ion

1.2. a segmented quadrupole mass filter with improved selectivity

1.3. ion transmission

2. Features:

2.1. novel high-sensitivity API interface

2.2. advanced active beam guide

2.3. advanced quadrupole technology

2.4. advanced vacuum technology

2.5. advanced peak determination (APD)

3. How to analyse protein:

3.1. needs to be digests into polypeptide by using an enzyme

3.2. trypsin was used as the enzyme

3.2.1. because it is the first choice for digestion readily available

3.3. polypeptide is inserted

3.4. the spectrometer will analyse the molecules

3.5. the detector will detect the polypeptide as single amino acid

3.5.1. each amino acid will have different mass

3.6. able to know the structure of protein from the peptides

4. Limitations:

4.1. sample needs to be in protein structure or conformation

4.2. sticky samples can block the tubes

4.3. high concentration of urea that may disturb the environment of mobile phase