Viruses Exam 1

1. Ch. 2 Protein Composition & Structure

1.1. The Amino Acids

1.2. Acid-base Properties

1.3. Peptide bonds & primary structure

1.4. Secondary Structure

1.5. Domains (x-tra)

1.6. Super Secondary Structure

1.7. Tertiary structure

1.8. Quaternary structure

1.9. Protein folding

1.10. Post-translational modifications

2. 3. Exploring Proteins

2.1. Protein Purification

2.1.1. 1. Ion exchange- separate by pI

2.1.2. at a pH of 9, Proteins A, C, F will come out last (Separate in 2 groups)

2.1.3. 2. Dialysis/filtration for mass, pore size will be 70kD and 30kD, proteins A & C will come out first and F will come out next and isolated.

2.1.4. Then I will use Hydroxyapetite to exclude by subunit between A &C

2.1.5. Lastly I will do Affinity Chromatography to separate B, D, from E where D will be bound since it was glycosylated and dilute filtrate one last time at 30kD to get B away from E

2.2. Immunology

2.3. Mass Spectrometry & Sequencing

2.3.1. w

2.3.2. d

2.4. Peptide synthesis

2.5. Determining 3-D Structures

3. Ch.5 Exploring Genes & Genomes

3.1. Tools for Molecular Bilolgy

3.2. Recombinant DNA

3.3. Complete Genomes

3.4. Manipulating Eukaryote Genes

4. 4. mDNA & RNA

4.1. Nucleic Acids

4.1.1. d

4.1.2. f

4.1.3. r

4.1.4. g

4.2. Double Helix

4.3. DNA Replication

4.4. Polymerases

4.5. Gene Expression

4.6. Genetic Code

4.7. Introns & Exons

5. Ch.1 Biochemistry

5.1. DNA, RNA, Proteins

5.2. Drug Design

5.3. Chemical & Non covalent Bonds

5.4. Thermodynamics

5.5. Functional Groups

6. Ch. 6 Evolution & Bioinformatics

6.1. Homologs

6.2. Statistics of Sequence Alignments

6.3. Structures & Evolution

6.4. Evolutionary Trees

6.5. Experimental Exploration of Evolution