1. Physical Method
1.1. Heat
1.1.1. Dry
1.1.1.1. Red Heat
1.1.1.1.1. Redness
1.1.1.2. Flaming
1.1.1.2.1. Without Redness
1.1.1.3. Incineration
1.1.1.3.1. Dead Bodies
1.1.1.4. Infra Red Rays
1.1.1.4.1. 180 C
1.1.1.5. Hot - Air Oven
1.1.1.5.1. Precaution
1.1.1.5.2. Uses
1.1.2. Moist
1.1.2.1. Below 100 C
1.1.2.1.1. Pasteurization of milk
1.1.2.1.2. Sterilization of serum
1.1.2.2. At 100 C
1.1.2.2.1. Boiling
1.1.2.2.2. Steaming
1.1.2.2.3. Tyndallization
1.1.2.3. Above 100 C (Autoclave)
1.1.2.3.1. Principles
1.1.2.3.2. Uses
1.2. Filtration
1.2.1. Uses
1.2.1.1. Toxin
1.2.1.2. Sera & Vaccine
1.2.2. Efficiency
1.2.2.1. Serratia marcescens / 0.75 um
1.2.3. Types
1.2.3.1. Asbestos paper
1.2.3.2. Cellulose membrane
1.3. Irradiation
1.3.1. UVR
1.3.2. Ionizing irradiation
1.3.2.1. Cobalt 60
2. Chemical Method
2.1. Gases
2.1.1. Formaldehyde
2.1.1.1. room disinfectant.
2.1.2. Ozone (O3):
2.1.2.1. sterilization of water.
2.1.3. Aerosol
2.1.3.1. Vaporized / room disinfectant.
2.1.4. Ethylene oxide:
2.1.4.1. Lethal to all kinds of microbes
2.2. Liquids
2.2.1. Chloroform
2.2.1.1. 0.5% used for preservation of sera.
2.2.2. Phenol group
2.2.2.1. Stool & Sputum
2.2.3. Lysol
2.2.3.1. surgical instruments .
2.2.4. Salts of heavy metals
2.2.5. Formalin
2.2.5.1. Germicidal & Wool & vaccines
2.2.6. Halogens
2.2.6.1. Iodine & Tincture iodine & Chlorine
2.2.7. Oxidizing agents
2.2.7.1. Potassium permanganate H2O2
2.2.8. Alcohol
2.2.8.1. Need Water