Histoplasma capsulatum
by amani awad
1. Macroscpoic
1.1. At 25°C Colonies are slow growing and granular to cottony in appearance. The color is white initially and usually becomes buff brown with age.
1.2. At 37°C Creamy, slowly growing, moist and yeast-like colonies are formed.
2. Microscopy
2.1. 1-hyaline septate hyphae.
2.2. 2-Early growth produces round to pyriform microconidia.
2.3. 3-tuberculate macroconidia.
3. treatment
3.1. For some people, the symptoms of histoplasmosis will go away without treatment. Itraconazole is antifungal medication treat severe histoplasmosis in the lungs, treatment can range from 3 months to 1 year.
4. sample:
4.1. taking a blood sample, bone marrow or a urine. may do imaging tests such as chest x-rays or CT scans of your lungs.They may also collect a sample of fluid from your respiratory tract or perform a tissue biopsy.
5. Direct examination
5.1. lactophenol cotton blue test.
6. Media:
6.1. Sabouraud agar; 1- Mold culture at 25°C 2- Yeast culture at 37°C
7. Conformatory tests
7.1. 1- DNA probe (AccuProbe, GenProbe Inc.)
7.2. 2-PCR
7.3. 3- immunoassay
