Vertebrate Eye Development

1. Embryonic stem cells

1.1. Self organizing

1.2. In vitro ES cultures

1.2.1. RPE cells derived into monolayers

1.2.2. Lens-like structures and retinal progenitor rosettes

1.2.2.1. eye tissue potential

1.2.3. Matrigel plus GFP

1.2.3.1. neuroepithelium-like layer

1.2.3.2. optic vessicle-like formation

1.2.3.3. synthetic cup forms

1.2.3.3.1. undergo cell differentiation

1.2.3.3.2. 3-D models to fight disease

1.2.4. ES derived retinal cells

1.2.4.1. Self patterning

1.2.4.1.1. Latent intrinsic order

1.3. Embryonic induction

1.3.1. interaction between surface ectoderm and optic vessicle

1.3.1.1. No optic cup = NO lens

2. eye field region

2.1. homogeneous pluripotent cells

2.1.1. evagination

2.1.1.1. invagination

2.1.1.1.1. bilayered cup