ESTABLISH "TILLING" METHOD FOR MALAYSIAN RICE IMPROVMENT
by Ali Talebi
1. Methods
1.1. Field Work
1.1.1. Seed Mutagenesis
1.1.1.1. irradiation
1.1.1.1.1. Optimizing Gamma Irradition
1.1.1.2. Optimizing Chemical Mutagenesis
1.1.1.2.1. EMS
1.1.2. Mutant Population M1,M2 and M3
1.2. Lab Works
1.2.1. Create Heteroduplexes
1.2.1.1. DNA Isolation
1.2.1.2. PCR Amplification
1.2.1.3. Create Heteroduplexes
1.2.1.4. Cleave mismatch products
1.2.1.5. Gel electrophoresis
1.2.1.6. Sequencing
1.2.2. Amino acid
1.2.2.1. HPLC
1.3. Analysis
1.3.1. Gel Image Analysis
1.3.2. Analyzing Mutant Individuals
2. MR 219
3. Design Primer
3.1. www.http://www.proweb.org/coddle
4. GIF1
5. TILLING (Targeting Induced Local Lesions IN Genomes)
6. started by:
6.1. RESEARCH GROUP
6.2. GENETICS
6.2.1. ALI BENJAVAD TALEBI
6.2.2. DR. CHE RADZIAH BINTI CEH MOHD ZAIN
6.2.3. DR. ZAMRI BIN ZAINAL
6.2.4. PROF. DR. MOHAMAD BIN OSMAN