1. Inoculation
1.1. Types of Media
1.1.1. physical
1.1.1.1. liquid
1.1.1.2. semisolid
1.1.1.2.1. agar
1.1.1.3. solid
1.1.2. chemical
1.1.2.1. defined
1.1.2.1.1. media whose chemical composition is chemical defined (known)
1.1.2.2. complex
1.2. Functional Types
1.2.1. reducing
1.2.1.1. contains substances that dissolves O2
1.2.2. selective
1.2.2.1. selects for 1 type
1.2.3. transport
1.2.3.1. maintains & preserves specimen
1.2.4. differential
1.2.4.1. visible differences
1.2.5. asay
1.2.5.1. test the effects to specific treatments to bacteria
1.2.6. carbohydrate fermentation
1.2.6.1. used as pH indicators
1.2.7. enumeration
1.2.7.1. isolate & identify (used in environmental & industrial microbiology)
1.3. Enriched
1.4. Complex Organic Substances
2. Incubation
2.1. temperature
2.1.1. preferred 20-45' celsius
2.2. gas
2.2.1. O2
2.2.2. CO2
3. Isolation
3.1. culture types
3.1.1. mixed culture
3.1.1.1. two or more, easily differentiate species of microorganisms
3.1.2. pure
3.1.2.1. one species
3.1.2.2. sub-culture
3.1.2.2.1. second level
3.1.3. contaminated
3.1.3.1. foreign bacteria infuriates medium
3.2. techniques
3.2.1. streak
3.2.1.1. a technique used to isolate stain from a single species of microorganisms
3.2.2. loop dilution
3.2.2.1. bacteria is diluted into tubes of melted algae
3.2.3. spread plate
3.2.3.1. a small volume of sample is pipetted into the buffer of the plate
3.2.4. pour plating
4. Inspection/Identification
4.1. microscopes
4.1.1. characteristics
4.1.1.1. light source
4.1.1.1.1. white light
4.1.1.1.2. uv light
4.1.1.2. magnification
4.1.1.3. contrast
4.1.1.4. resolution
4.1.2. types of
4.1.2.1. dark field
4.1.2.2. bright-field
4.1.2.3. face contrast
4.1.2.4. transmission electron
4.1.2.5. atomic force
4.1.2.6. scanning tunneling
4.1.2.7. scanning electron
4.1.2.8. confocal
4.1.2.9. interference
4.1.2.10. fluorescent
4.2. reference book
4.2.1. need informations
4.2.1.1. tests
4.2.1.1.1. biomedical
4.2.1.1.2. genotype
4.2.1.1.3. phenotype
4.2.1.1.4. staining
4.2.1.1.5. microscopic
4.2.1.1.6. macroscopic
4.2.1.1.7. immunological