Toolkit - Field Entomology

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Toolkit - Field Entomology by Mind Map: Toolkit - Field Entomology

1. Biobanking

1.1. Explainer: what is biobanking

1.2. Resources

1.2.1. Guideline: Biobanking

1.2.2. SOP: Biobanking field samples

1.2.3. Template: Biobanking field samples

1.2.4. SOP: Biobanking colony samples

1.2.5. Template: Biobanking colony samples

1.3. Case studies

1.3.1. 363 - Biobanking for DNA and RNA analysis

1.3.2. 364 - Biobanking samples for microscopy

1.4. Troubleshooting

1.5. How to reduce your biobanking freezer space

1.6. How to store squash buffer extraction plates

2. Mosquito collection

2.1. Explainer: types of traps

2.1.1. Fact sheet: Mosquito traps

2.1.2. 149: Choosing the right trap

2.1.3. 286. Surveying mosquitoes in Fiji

2.2. BG traps

2.2.1. Resources

2.2.1.1. SOP: Mosquito collection using BG traps

2.2.1.2. How to use the Field App for BG trap runs

2.2.1.3. Example: BG trap fact sheet

2.2.2. Stories

2.2.2.1. 274 Installing BG traps in Yogya

2.2.2.2. 386. Setting up BG2 traps

2.2.2.3. 13. Servicing a BG trap in Cairns

2.2.2.4. 156 Managing traps in Nha Trang

2.2.2.5. 345. Using ODK (Field App) for monitoring

2.2.2.6. 425. Searching for BG trap hosts

2.2.2.7. 432. Deploying traps in La Paz

2.2.3. Troubleshooting

2.2.3.1. Troubleshooting guide: BG traps

2.2.3.2. 3054. Reaching people in high rise buildings

2.2.3.3. 323. Reducing BG trap collections

2.2.4. Links to activities

2.2.4.1. Entomology survey [FE]

2.2.4.2. IR survey [FE]

2.2.4.3. Set up baseline monitoring network [FE]

2.3. Prokopacks

2.3.1. Stories

2.3.1.1. 3052. Using Prokopacks in Colombia

2.3.2. Resources

2.3.2.1. SOP: Mosquito collection using Prokopacks

2.3.2.2. Example: Prokopack fact sheet

2.3.3. Troubleshooting

2.3.3.1. Troubleshooting guide: Prokopacks

2.4. Ovitraps

2.4.1. Stories

2.4.1.1. 158. Ovitraps in Townsville

2.4.1.2. 390. Collecting wildtype mosquitoes for outcrossing

2.4.2. Resources

2.4.2.1. SOP: Mosquito collection using ovitraps

2.4.2.2. How to use the Field App for ovitrap runs

2.4.2.3. How to calculate the number of ovitraps required

2.4.2.4. Example: ovitrap fact sheet

2.4.3. Troubleshooting

2.4.3.1. Troubleshooting guide: Ovitraps

2.4.4. Links to activities

2.4.4.1. Collect wildtype mosquitoes for backcrossing [FE]

2.4.4.2. insecticide resistance [FE]

2.5. Innovation/Scale up

2.5.1. What would the ideal trap do?

2.5.1.1. exchange

2.5.2. Rolling deployment during large scale release

2.5.3. What to do when you can't find mosquitoes

2.6. Training

2.7. Exchange

3. Working in the community

3.1. Explainer: why is CE important for field teams

3.1.1. 26. Embracing Engagement

3.2. Resources

3.2.1. Guideline: Staff induction training

3.2.2. Guideline: Hiring and training FE staff

3.2.3. Global messaging

3.2.4. example Fact Sheets (WMP, Wolbachia, traps)

3.3. Stories

3.3.1. 6010. Early CE training for the field team.

3.3.2. 88. Tri Nguyen's advocay team - engaging community stakeholders

3.3.3. 172. Community facilitators in Paris - local volunteers used for CE activities

3.3.4. 26. Embracing Engagement

3.3.5. 237. Building a community engagement team

3.3.6. 387. Working in dangerous areas

3.3.7. 398. Staying safe in Medellin

3.3.8. 274. Installing BG traps in Yogyakarta

3.3.9. 39. Print matters

3.3.10. 7014. PAM (explainer)

3.4. Troubleshooting

3.4.1. 65: Extreme weather

3.4.2. 3054. Reaching people in high-rise buildings

3.4.3. 387. Working in dangerous areas

3.4.4. 365. Involving all team members in community engagement

3.4.5. 3004. Removing BG traps afer years of engagement

3.5. Links to activities

3.5.1. Entomology survey [FE]

3.5.2. Monitoring [FE]

3.5.3. Egg releases [FE]

3.5.4. Stakeholder engagement [PC]"

3.6. Innovation/Scale up

3.6.1. Finding BG hosts in complex cities

3.6.2. Effective community engagement at scale

3.6.3. Involving the community in releases

3.7. Training

3.8. Exchange

4. Insecticide resistance

4.1. Introduction

4.2. 80. Improving insecticide resistance

4.3. IR surveys

4.3.1. Stories

4.3.1.1. 255. Mapping insecticide resistance across Nha Trang.

4.3.2. Resources

4.3.2.1. Guidelines: Surveying IR in Aedes aegypti populations

4.3.2.2. Online tool: IR mapper (for mapping and viewing the insecticide resistance of mosquito populations)

4.4. IR tests – bioassays

4.4.1. Resources

4.4.1.1. Guidelines: Monitoring and managing insecticide resistance in Aedes mosquito populations (WHO)

4.4.1.2. SOP: Test procedures for insecticide resistance monitoring in malaria vector mosquitoes (WHO)

4.4.1.3. SOP: Testing insecticide resistance by bioassay

4.4.1.4. Template: IR graph

4.4.2. Troubleshooting

4.4.2.1. 3042. A bioassay tube trick

4.5. IR tests – kdr tests

4.5.1. Resources

4.5.1.1. SOP: Testing insecticide resistance by kdr qPCR (96-well)

4.5.2. Stories

4.5.2.1. 3033. Kdr tests for insecticide resistance

4.6. Links to activities (TBC)

4.6.1. Gather information on local entomology [FE]

4.6.2. IR survey [FE]

4.6.3. Post-backcrossing health check of release colony [MP]

4.6.4. Pre-release health check of release colony [MP]

5. Core Data

5.1. see Data & Digital Toolkit

6. Process field samples

6.1. see Mosquito Production Toolkit

7. Mosquito releases

7.1. Introduction

7.1.1. 333. Comparing adult to egg releases

7.1.2. 369. Adults or eggs?

7.2. Planning a release

7.2.1. Stories (refining & mapping release area)

7.2.1.1. 355 Ground-truthing release maps

7.2.1.2. 340. Choosing release sites

7.2.1.3. 277. Scoping a new release site

7.2.1.4. 398. Staying safe in Medellin

7.2.1.5. 220 Simple release maps

7.2.1.6. 332 Releasing mosquitoes on a grid

7.2.1.7. 413 Planning vehicle releases

7.2.2. Stories (planning & managing release)

7.2.2.1. 212. Managing the logistics of a field trial

7.2.2.2. 213. Managing the daily tasks of a large team

7.2.2.3. 266 Planning community releases

7.2.2.4. 303 Practice Release

7.2.3. Resources

7.2.3.1. Guidelines: Developing a release and monitoring plan

7.2.3.2. Guidelines: Community rmosquito releases

7.2.3.3. Guidelines: Practice release

7.2.4. Links to activities

7.2.4.1. Practice release [PC+FE]

7.2.4.2. Release [FE]

7.3. Releasing mosquito eggs

7.3.1. Stories

7.3.1.1. 265 Adapting to eggs

7.3.1.2. 19. Developing MRCs

7.3.1.3. 125. How to make an MRC

7.3.1.4. 83. How to deploy an MRC

7.3.1.5. 411. Servicing mosquito release containers

7.3.1.6. 372. Checking hatch rates in release containers

7.3.1.7. 373. Deploying MRCs in public areas

7.3.1.8. 117. Rapid deployment, city-wide

7.3.1.9. 94. Engaging school children to release mosquitoes

7.3.1.10. 136. Transporting eggs

7.3.2. Troubleshooting

7.3.2.1. 177. Quality assurance for egg releases

7.3.2.2. 347. Moving mosquito release containers

7.3.2.3. 124. Hatching trouble

7.3.3. Resources

7.3.3.1. Guide: Choosing a mosquito release container

7.3.3.2. SOP: Releasing mosquitoes - Eggs

7.3.3.3. SOP: Using Field app for egg release runs

7.3.3.4. Example: MRC label

7.3.4. Links to activities

7.4. Releasing adult mosquitoes

7.4.1. Stories

7.4.1.1. 334 Releasing adult mosquitoes in Charters Towers

7.4.1.2. 338 Choosing a cup for rearing and releases

7.4.1.3. 337 Rearing mosquitoes for release

7.4.1.4. 315. Simple rearing for release

7.4.1.5. 3003. Finding the right cup for adult releases

7.4.1.6. 3009. Adapting adult release cups

7.4.1.7. 3063. Improving release bags

7.4.1.8. 3064. Improving motorbike releases

7.4.2. Troubleshooting

7.4.2.1. 359. Investigating fail rates in adult release cups

7.4.2.2. 3059. Honey for release tubes

7.4.3. Resources

7.4.3.1. Guide: Choosing an adult release container

7.4.3.2. SOP: Releasing mosquitoes - Adults

7.4.3.3. SOP: Preparing adult mosquitoes for remote release (cupping)

7.4.3.4. SOP: Using Field app for adult release runs

7.4.4. Links to activities

7.5. Innovation/Scale up

7.5.1. Large scale release

7.5.2. Involving the community in releases

7.5.3. Drones