Yeast and E. coli in DDW Project Outline

1. General Project Plan

1.1. E Coli

1.1.1. OD measurments of growth in DDW, D2O, and H2O

1.1.2. microscope observations

1.1.2.1. general observations

1.1.2.2. observations through staining

1.2. Yeast

1.2.1. OD measurements of growth in DDW, D2O, and H2O

1.2.1.1. asynchronous measurements

1.2.1.2. synchronized growth

1.2.1.2.1. need mating pheromone

1.2.2. microscope observations

1.2.2.1. asynchronous observations

1.2.2.2. synchronized observations

2. Preparation

2.1. Growth

2.1.1. get reacquainted with growing cultures

2.1.2. figure out ways to prepare growth media optimally

2.2. Nanodrop

2.2.1. learn how to measure OD