1. Chemical Method
1.1. Gases
1.1.1. Formaldehyde
1.1.1.1. room disinfectant.
1.1.2. Ozone (O3):
1.1.2.1. sterilization of water.
1.1.3. Aerosol
1.1.3.1. Vaporized / room disinfectant.
1.1.4. Ethylene oxide:
1.1.4.1. Lethal to all kinds of microbes
1.2. Liquids
1.2.1. Chloroform
1.2.1.1. 0.5% used for preservation of sera.
1.2.2. Phenol group
1.2.2.1. Stool & Sputum
1.2.3. Lysol
1.2.3.1. surgical instruments .
1.2.4. Salts of heavy metals
1.2.5. Formalin
1.2.5.1. Germicidal & Wool & vaccines
1.2.6. Halogens
1.2.6.1. Iodine & Tincture iodine & Chlorine
1.2.7. Oxidizing agents
1.2.7.1. Potassium permanganate H2O2
1.2.8. Alcohol
1.2.8.1. Need Water
2. Physical Method
2.1. Heat
2.1.1. Dry
2.1.1.1. Red Heat
2.1.1.1.1. Redness
2.1.1.2. Flaming
2.1.1.2.1. Without Redness
2.1.1.3. Incineration
2.1.1.3.1. Dead Bodies
2.1.1.4. Infra Red Rays
2.1.1.4.1. 180 C
2.1.1.5. Hot - Air Oven
2.1.1.5.1. Precaution
2.1.1.5.2. Uses
2.1.2. Moist
2.1.2.1. Below 100 C
2.1.2.1.1. Pasteurization of milk
2.1.2.1.2. Sterilization of serum
2.1.2.2. At 100 C
2.1.2.2.1. Boiling
2.1.2.2.2. Steaming
2.1.2.2.3. Tyndallization
2.1.2.3. Above 100 C (Autoclave)
2.1.2.3.1. Principles
2.1.2.3.2. Uses
2.2. Filtration
2.2.1. Uses
2.2.1.1. Toxin
2.2.1.2. Sera & Vaccine
2.2.2. Efficiency
2.2.2.1. Serratia marcescens / 0.75 um
2.2.3. Types
2.2.3.1. Asbestos paper
2.2.3.2. Cellulose membrane
2.3. Irradiation
2.3.1. UVR
2.3.2. Ionizing irradiation
2.3.2.1. Cobalt 60