Directed Enzyme Evolution
by Wesley Barrios
1. New C-X bonds are waiting
2. Cyclopropenes
3. Birth of the Field
3.1. 2001
4. Frances H. Arnold
5. But how enzymes WORK?
6. What are enzymes?
7. DNA: We can READ
8. DNA: We can WRITE
9. DNA: We can EDIT
10. DNA: We don't know how to compose
11. A multiverse of possibilities
12. NATURE has the recipe
12.1. Evolution
12.2. Solved ALL the problems of beeing alive
13. Maynard, 1970
14. Directed Evolution
15. Diversification of sequences
16. Re-Optimization
17. Examples
18. Examples
19. Novelty is HERE
20. Frances Lab
21. Bycyclopropenes
22. What about Si-C bonds?
23. It occurs in nature!
24. Unnatural Selection
25. Screening
26. A multiverse of possibilitities
27. 10^24 Stars in the Universe
28. Nitrene transfer
29. Enzyme Tuning
30. References GRIESBECK, O. CRISPR/Cas9-based directed evolution in mammalian cells. Current Opinion in Structural Biology, v. 69, p. 35–40, ago. 2021. BUNZEL, H. A.; ANDERSON, J. L. R.; MULHOLLAND, A. J. Designing better enzymes: Insights from directed evolution. Current Opinion in Structural Biology, v. 67, p. 212–218, abr. 2021. FARINAS, E. T.; BULTER, T.; ARNOLD, F. H. Directed enzyme evolution. Current Opinion in Biotechnology, v. 12, n. 6, p. 545–551, dez. 2001. BASU, S. et al. A synthetic multicellular system for programmed pattern formation. Nature, v. 434, n. 7037, p. 1130–1134, abr. 2005. Setting the stage for evolution of a new enzyme. WITTMANN, B. J. et al. Advances in machine learning for directed evolutionCurrent Opinion in Structural BiologyElsevier Ltd, , 1 ago. 2021. ARNOLD, F. H. Innovation by Evolution: Bringing New Chemistry to Life (Nobel Lecture)Angewandte Chemie - International EditionWiley-VCH Verlag, , 7 out. 2019. ENGQVIST, M. K. M.; RABE, K. S. Applications of Protein Engineering and Directed Evolution in Plant Research 1[OPEN]. Update on Protein Engineering and Directed Evolution , 2020.
31. Plant Applications
32. Perspectives
