Directed Enzyme Evolution

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Directed Enzyme Evolution by Mind Map: Directed Enzyme Evolution

1. New C-X bonds are waiting

2. Cyclopropenes

3. Birth of the Field

3.1. 2001

4. Frances H. Arnold

5. But how enzymes WORK?

6. What are enzymes?

7. DNA: We can READ

8. DNA: We can WRITE

9. DNA: We can EDIT

10. DNA: We don't know how to compose

11. A multiverse of possibilities

12. NATURE has the recipe

12.1. Evolution

12.2. Solved ALL the problems of beeing alive

13. Maynard, 1970

14. Directed Evolution

15. Diversification of sequences

16. Re-Optimization

17. Examples

18. Examples

19. Novelty is HERE

20. Frances Lab

21. Bycyclopropenes

22. What about Si-C bonds?

23. It occurs in nature!

24. Unnatural Selection

25. Screening

26. A multiverse of possibilitities

27. 10^24 Stars in the Universe

28. Nitrene transfer

29. Enzyme Tuning

30. References GRIESBECK, O. CRISPR/Cas9-based directed evolution in mammalian cells. Current Opinion in Structural Biology, v. 69, p. 35–40, ago. 2021. BUNZEL, H. A.; ANDERSON, J. L. R.; MULHOLLAND, A. J. Designing better enzymes: Insights from directed evolution. Current Opinion in Structural Biology, v. 67, p. 212–218, abr. 2021. FARINAS, E. T.; BULTER, T.; ARNOLD, F. H. Directed enzyme evolution. Current Opinion in Biotechnology, v. 12, n. 6, p. 545–551, dez. 2001. BASU, S. et al. A synthetic multicellular system for programmed pattern formation. Nature, v. 434, n. 7037, p. 1130–1134, abr. 2005. Setting the stage for evolution of a new enzyme. WITTMANN, B. J. et al. Advances in machine learning for directed evolutionCurrent Opinion in Structural BiologyElsevier Ltd, , 1 ago. 2021. ARNOLD, F. H. Innovation by Evolution: Bringing New Chemistry to Life (Nobel Lecture)Angewandte Chemie - International EditionWiley-VCH Verlag, , 7 out. 2019. ENGQVIST, M. K. M.; RABE, K. S. Applications of Protein Engineering and Directed Evolution in Plant Research 1[OPEN]. Update on Protein Engineering and Directed Evolution , 2020.

31. Plant Applications

32. Perspectives