Drosophila melanogaster Sex comb reduced (Scr)

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Drosophila melanogaster Sex comb reduced (Scr) by Mind Map: Drosophila melanogaster Sex comb reduced (Scr)

1. Scr gene

1.1. Species: Drosophila melanogaster

1.2. Gene family: Homeobox (Hox) proteins

1.3. Tissue specificity gene expression :

1.3.1. Embryo: labial & anterior prothoracic segments & extreme posterior of the maxillary segment Denticle belt formation and fusion of labial lobes

1.3.2. Adults: eye maxillary analgen, antennal imaginal disk, labial and T1 leg imaginal disks

1.4. Protein product: Homeotic protein Sex comb reduced (Scr)

1.4.1. Function: DNA binding transcription factor

1.4.2. Function: developmental protein

1.4.3. PTM: phosphoprotein (S216)

1.4.4. Length: 417 amino acids

1.4.5. Structure: Mostly helices, with an Antp-type hexapeptdie motif

1.4.6. Subtypes: RA, RD, RE, RF, RB

1.5. Development of salivary glands and sex comb dependent on Scr expression

2. Question(s)

2.1. How could Scr gene expression affect sex comb morphology?

2.1.1. Bristle numbers?

2.1.2. Symmetry

2.1.3. Size

2.2. Could the manipulated sex comb morphology (result of Scr expression manipulation) have an effect on sexual selection?

2.2.1. male-male competition

2.2.2. female choice based on sex comb phenotype

2.2.3. variation of sex comb phenotype increase decrease

3. Methods

3.1. Introduce mutation(s) to Scr gene via vector

3.1.1. Use the five hypomorphic mutations described in earlier studies Scr1 Scr3 Scr5 Scr6 Scr8

3.2. Or modify Scr protein expression via PTM at N-terminal homeodomain arm

3.2.1. Desphosphorylation Active Scr

3.2.2. Phosphorylation Inactive Scr

3.3. Measure Sex comb morphologies (bristle numbers, length, size, symmetry and shape

3.4. Plot mutations, sex comb morphologies and reproductive success of males and determine if correlations are statistically significant

4. Sex comb

4.1. Secondary, dimorphic sex organ

4.2. Morphology correlated with male reproductive success

4.2.1. bristle numbers

4.2.2. size

4.2.3. symmetry

4.2.4. absent/ present

4.3. Used to grab female's abdomen and genitalia during copulation