CULTURE AND CULTURE MEDIA

1. 4. Differential media

1.1. allow the visualization of metabolic differences

1.2. Examples: MAC, BAP, eosin methylene blue (EMB), and Hektoen enteric agar (HEA)

1.2.1. MAC - differentiates lactose fermenters (pink colonies) from non lactose fermenters (colorless colonies)

1.2.2. Neutral red - pH indicator in MAC

2. 5. Selective media

2.1. antibiotics, dyes, or chemicals to inhibit the growth of other organisms while promoting the growth of the desired organism

2.2. Examples: HEA, MAC, xylose lysine desoxycholate (XLD) agar, bismuth sulfite agar (BSA), mannitol salt agar (MSA), and Thayer-Martin agar (TMA)

3. 6. Special media

3.1. used to isolate bacteria with specific growth requirements

3.2. Examples: Lowenstein-Jensen (LJ) medium and thiosulfate-citrate-bile salts-sucrose (TCBS) agar.

4. 1. Simple media, general purpose media, and supportive media

4.1. routinely used in the laboratory

4.2. without additional supplements

4.3. support the growth of non fastidious bacteria

4.4. composed of meat and soybean extracts

4.5. Examples: Nutrient Agar (NA), Nutrient Broth (NB), and TSB

5. 2. Enrichment media (Liquid-type media)

5.1. specific nutrients

5.2. without additional supplements

5.3. detect aerobes, anaerobes, and microaerophiles (thioglycollate)

5.4. Examples: alkaline peptone water, selenite F, thioglycollate, tetrathionate. Gram negative (GN) broth, and Lim broth

6. 3. Enriched media and non-selectived media

6.1. with additional supplements

6.2. growth of fastidious organism

6.3. solid-type media

6.4. Examples: BAP and CAP

6.4.1. BAP - differentiate hemolytic patterns of bateria

6.4.1.1. CAP - recovery of Haemophilus species