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E Coli DNA by Mind Map: E Coli DNA
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E Coli DNA

Restriction / Modification System

Modification Methylase

topoisomerases

Replication

preconditions

@oric

polymerization

replication errors

terms

termination

postconditions

Structure&Topology

pyrimidine

purine

major/minor groove

topoisomerases

conformations: b, a, z DNA

bdna has 10bp/turn and is the "typical" conformation.

Linking = twists + writh (heteroduplex)

phosphodiester

sugar-phosphate

Analysis Techniques

Gel Electrophoresis

restriction site mapping

DNA damage & repair

Replication Errors

spontaneous

chemical damage

UltraViolet Radiation

Repair

Nucleotide Excision Repair (NER)

Base Excision Repair

Adaptive Response "methyl group repair"

DNA repair involving no lesion removal

      

unresolved

primosome assembly

"minutes" vs 84 degrees/minutes

Recombination

Rec BCD Pathway

holiday junction

RecA protein & pathway