Bio-Phage Flow Chart

1. Collect soil sample- choose soil that is slightly moist

1.1. Direct plating: To isolate a phage from a soil sample

1.2. Enrichment: This method helps to increase the chance of recovering a phage by increasing the number of phages

1.2.1. Spot Test: Determining whether there is plaque or contamination

1.2.1.1. Picking a plaque: To obtain a liquid sample of phage from a plaque on a lawn of bacteria

1.2.1.1.1. Plaque Purification: To obtain a lysate of only one type of phage