Measure activity

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Measure activity by Mind Map: Measure activity

1. producing some clean knowledge

2. Detect activity in single neurons/ brain areas (as reliably as possible and as fast as possible)

3. Reporters

3.1. GCAMP3

3.2. GCAMP6

3.3. Arclight

4. Exp details

4.1. Saline

4.2. Dissections

4.2.1. Seelig2010

4.2.2. Kohatsu2011

4.2.3. Opening the front of the head by removing antennae, and associated cuticle to see SOG

4.2.4. takeo stechnique

4.3. excitation

4.3.1. laser (488nm, 50mW)

4.3.1.1. get quote

4.4. stage

4.5. study other methods

4.6. optics

4.6.1. clean up

5. paradigms

5.1. Have a paradigm that works well

5.1.1. Odor

5.1.1.1. GCAMP3 experiments

5.2. pdf

5.2.1. pdf nachbac troubleshooting

5.3. Tsien lab dyes

5.4. reward anticipation

5.5. any activity TH

5.5.1. TH-arclight x arclight

5.5.2. very clean trials GCAMP6

5.5.3. effect of temperature

6. Analysis

6.1. ICA with Eran s program

6.2. variance asymetry

6.3. Kalman filters

7. Problems

7.1. Slow shifts in fluorescence

7.1.1. unstable chamber

7.1.2. water under objective

7.1.3. proboscis

7.1.4. lamp

7.2. Movements

7.2.1. cut muscle 16

7.2.1.1. figure out how people do it

7.2.2. fix the fly

7.3. No activity detected reproducibly

7.3.1. solved

7.3.1.1. pdf flies shouldn t be put into ice

7.3.1.2. bad saline

7.3.2. problem with GH flies

7.3.2.1. try simple GCAMP3

7.3.3. other general problem

7.3.3.1. GCAMP3 30Y

7.3.4. problem with odor delivery

7.3.4.1. contamination

7.3.4.2. too high odor

7.4. Background fluorescence

7.4.1. presence of probocsis

7.4.2. food

7.4.3. filters!

7.5. side problems

7.5.1. effect of evaporation

7.6. Archives

7.6.1. Identify neurons

7.6.1.1. too high background

7.6.1.2. too many artefacts (fat bodies...)

7.6.1.3. too low expression

7.6.1.4. not enough dynamics (=> 16 bits)

7.6.2. weird GCAMP6FVK?

7.6.2.1. red weird stuffs

7.6.2.2. no fluo